Fig. 6.
Comparison of ERK activity reporters. (A) EGF-induced changes in the acceptor-to-donor fluorescence ratio in HEK293 cells expressing CFP-YFP versions of EKARcyto, Erkuscyto, or Miu2. Example traces from individual experiments are shown. (B) Ratio changes for CFP-YFP versions of EKARcyto, Erkuscyto, and Miu2 in HEK293 cells after EGF application. The ratio, R, was measured as the YFP/CFP ratio for EKARcyto and the CFP/YFP ratio for Erkuscyto and Miu2. Data are mean ± SEM for ≥5 ROIs from 5 dishes.