Fig. 2.

APP expression affects mitochondrial fusion. M17 cells were transfected with mito-Dendra2 to label mitochondria. Before photo-conversion (Pre), Dendra2 emits green fluorescence. At time 0, laser activation is applied to ROI (square box) to allow full photo-conversion, from green to red, of all of the mitochondria within the ROI. Thereafter, mitochondrial behavior in the entire cell was monitored for 60 min. Active mitochondria fission (filled arrowhead) and fusion (open arrowhead) of individual mitochondrion could be observed in the representative control cell. (Scale bars, 20 μm.)