Fig. 2.

Tissues of expression of mating responsive genes. Schematic representation of a whole female mosquito, showing the tissues examined by qRT-PCR and the genes detected in each tissue, listed in the insets of the corresponding color. The tissues tested were the whole head (head capsule and brain), the gut (midgut, hindgut, and rectum), the ovaries, the LRT (atrium, spermatheca, and parovarium), and the carcass (cuticle, muscle, and fat body). A schematic representation of the female reproductive tract is also provided, showing the atrium (yellow) and the spermatheca (brown). The ovaries and the parovarium are greyed as they were not analyzed further. In bold are genes that are primarily expressed in 1 tissue (at least 10-fold higher than in any other tissue, after correcting for control gene levels). For genes expressed in the LRT and gut, mean fold changes from 3 biological replicates are indicated (Fold change) and the time point showing the largest change (Time, h). The levels of expression of each gene in virgins (V) and at 6 h and 24 h, normalized against the control gene RpL19, are indicated by a color gradient (see bar). The last column in the LRT inset indicates whether a gene was expressed primarily in the atrium (A) or spermatheca (S). All changes are significant at the level of P < 0.05 unless otherwise indicated (ns). †, also down-regulated at 24 h (−3.9-fold); ‡, also up-regulated at 24 h (73.3-fold).