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. 2008 Dec 3;105(49):19450–19455. doi: 10.1073/pnas.0809879105

Fig. 2.

Fig. 2.

Neutralization assays with HCVcc or HCVpp containing mutations in E2 conferring resistance to AP33 neutralization. (A and B) The N415Y and E655G mutations were inserted into pHJ3–5 plasmid DNA singly or in combination to generate virus with defined amino acid substitutions. N415Y (open triangles), E655G (open squares), and N415Y+E655G (open circles) mutants and wt (filled diamonds) virus were analyzed for resistance to neutralization by various concentrations of (A) AP33 or (B) HC-1 mAbs by FFU reduction assay. Error bars indicate range in replicate experiments. (C and D) HCVpp containing either wt H77c envelope protein (filled diamonds) or N415Y (open triangles), E655G (open squares), and N415Y+E655G (open circles) mutants were tested for resistance to neutralization by various concentrations of (C) AP33 or (D) HC-1 mAbs. Neutralization is expressed as percent reduction (±SD) in luciferase activity.