Fig. 5.
Translation of full-length ndhC/K/J mRNAs. (A) Schematic representation of the tricistronic mRNAs. Asterisks indicate ndhC termination sites. (B) Gel pattern of products. Translation was performed with fluorescent fMet-labeled tRNAfmet. Products were separated on denatured gels. The calculated kDa of NdhC, NdhK (S3), and NdhJ are 13.9, 28.0, and 18.6, respectively. Faint bands above and below NdhK are those from S1 (32.3 kDa) and S4 (25.5 kDa), respectively. A band between NdhJ and NdhC in lane mT1 is an extended NdhC (16.4 kDa). Size markers are fluorescent (FITC)-labeled molecular mass marker (APRO Life Science Institute). (C) Translation efficiencies are as in Fig. 1D. Only the major NdhK from S3 was used.