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To quantify platelet derived microparticles, debris from the coronary sinus that was gated as less than 1 μm in size by flow cytometry was sorted for the constitutive platelet marker, CD41, and the activation marker, annexin V. These representative histograms illustrate that a higher proportion of the subcellular debris from the coronary sinus of patients after OPCAB was derived from activated platelets compared with a smaller proportion in the sample obtained after on-pump CABG.
