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. 2009 Jan 12;4(1):e4170. doi: 10.1371/journal.pone.0004170

Figure 1. Selective and specific activation of PERK signaling.

Figure 1

(A) Parental wild-type and transgenic HEK293 cells expressing the AP20187-sensitized Fv2E-Perk allele were treated for the indicated times with AP20187 (2 nM). Fv2E-Perk, Gadd34, and Chop mRNA levels were measured by quantitative PCR, normalized to levels of a housekeeping gene, Rpl19, and are shown relative to levels in untreated cells. Fv2E-PERK, phospho-eIF2α, and ATF4 proteins were detected by immunoblotting. Total eIF2α protein was measured as a loading control. (B) Parental wild-type and transgenic HEK293 cells expressing the AP20187-sensitized Fv2E-Perk allele were treated for the indicated times with AP20187 (2 nM). Xbp1 mRNA splicing was assesed by RT-PCR. The unspliced (u) and spliced (s) Xbp1 mRNA products are indicated as labeled. The asterisk indicates the position of a hybrid amplicon.