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. Author manuscript; available in PMC: 2009 Jan 7.
Published in final edited form as: J Mol Biol. 2008 May 13;380(2):278–284. doi: 10.1016/j.jmb.2008.05.015

Figure 1.

Figure 1

MarA molecules per cell as a function of IPTG concentration in wild type and lon− clpP−cells. The inset is one of many Western blots from which the values in the graph were calculated. As previously noted,12 MarA made in vivo migrates slightly faster than the purified material from the plasmid construct which contains an additional 5 amino acids at its N-terminus. There is a band also present with pre-immune serum that moves slightly less rapidly than the MarA. Each lane contained the extract from 4.5*107 lon− clp− cells (M3897) or 1.4*108 wild-type cells (M3723). The two MarA lanes contained 11 (left lane) and 33 ng (right lane) of purified MarA.