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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Hepatology. 2008 Nov;48(5):1396–1403. doi: 10.1002/hep.22508

Figure 5. Inhibition of FASN blocks HCVpp entry.

Figure 5

(A, B) Huh7 cells were treated with indicated concentration of C75 for 12 hours (hrs) and then spin-infected with HCVpp or VSVpp and incubated for additional 2 days followed by a luciferase assay to quantify virus entry. (C, D) Huh7 cells were transduced with 3 shRNAi lentiviruses (3216, 3218, and 3219) targeting FASN and the control shRNAi virus (Ctrl) respectively. Two days after lentiviral transduction, the cells were spin-infected with HCVpp or VSVpp and further incubated for 48 hrs prior to luciferase assay. Error bars represent standard deviations.