Figure 10.
Effects of Opuntia polysaccharides on NF-κB activation. Human THP-1 monocytes (5×105 cells/well) were incubated for 24 hr with Opuntia polysaccharide fractions C-I (□), C-I pretreated with endotoxin-removing gel (■), C-II (●), C-III (○), C-VI (◆), or 100 ng/ml LPS (◇, positive control). Alkaline phosphatase activity was analyzed spectrophotometrically (absorbance at 655 nm) in the cell supernatants, as described. Values are the mean ± S.D. of triplicate samples from one experiment, which is representative of two independent experiments. Statistically significant differences (P<0.05) between untreated cells and cells treated with fractions C-I (‡, *), C-I pretreated with endotoxin-removing gel ( ‡, *), C-II (‡, *), C-III (‡, *), and C-IV (*) are indicated.