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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Int Immunopharmacol. 2008 Jul 1;8(10):1455–1466. doi: 10.1016/j.intimp.2008.06.003

Figure 3.

Figure 3

Effect of Opuntia polysaccharides on murine macrophage ROS and NO production. Murine J774.A1 macrophages were incubated for 24 hr with the indicated concentrations of polysaccharide fractions C-I (□), -II (●), C-III (○), C-VI (◆), or 100 ng/ml LPS (◇, positive control). NO production was quantified by measuring nitrite in the cell-free supernatants (Panel A), and chemiluminescence was monitored using an L-012 detection system, as described (Panel B). Values are the mean ± S.D. of triplicate samples from one experiment, which is representative of two independent experiments. Statistically significant differences (P<0.05) between untreated cells and cells treated with fractions C-I (†, ‡, *), C-II (†, ‡, *), C-III (‡, *), and C-IV (*) are indicated.