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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Int Immunopharmacol. 2008 Jul 1;8(10):1455–1466. doi: 10.1016/j.intimp.2008.06.003

Figure 7.

Figure 7

Effect of Opuntia polysaccharides on macrophage TNF-α production. Murine J774.A1 (Panel A) or human MonoMac6 (Panel B) macrophages were incubated for 24 hr with the indicated concentrations of polysaccharide fractions C-I (□), C-I pretreated with endotoxin-removing gel (■), (C-II (●), C-III (○), C-VI (◆), or 50 ng/ml LPS (◇, positive control). Cell-free supernatants were collected, and extracellular TNF-α was quantified by ELISA. Values are the mean ± S.D. of triplicate samples from one experiment, which is representative of three independent experiments. Statistically significant differences (P<0.05) between untreated cells and cells treated with fractions C-I (†, ‡, *), C-I pretreated with endotoxin-removing gel (†, ‡, *), C-II (‡, *), C-III (*), and C-IV (‡, *) are indicated.