Figure 4.

WSSV403 can interact with a shrimp protein phosphatase. WSSV403 was found to interact with shrimp PPs in yeast two hybrid. Cotransformed yeast was screened on -Leu-Trp SD plates (A) and -Leu-Trp-His-Ade plates with x-α-gal (B). 1, yeast cotransformed with pGBK-403 and pGAD-PPs; 2, yeast cotransformed with pGBK-403 and pGAD; 3, yeast cotransformed with pGBK and pGAD-PPs; 4, yeast cotransformed with positive plasmids from the kit (Clontech). (C) Pull-down assays with WSSV403 and GST-PPs. Soluble protein complexes were bound to GST beads and washed under high stringency condition before SDS-PAGE and immunoblot detection of His₆-WSSV403. Immunoblots were performed with anti-His₆ or anti-GST.