Fig 7. FAF1 expression potentiates caspase 3 activation and apoptotic cell death.
FAF1 overexression by itself does not activate caspase 3 however in presence of proteasomal inhibitor lactacystin (10µm, 16hr) FAF1 can potentiate caspase 3 activation and therefore apoptotic cell death. Minimal cleaved caspase 3 (17–19kd bands) is detected in HEK cells with vector alone or with lactacystin treatment or with FAF1 overexpression. The red bands denote β-actin as a loading control. * p<0.05 control cells versus FAF1 or lactacystin-treated cells. Statistical significance was determined using multivariate ANOVA.