Table 1.
Gene Ontology analysis and KEGG pathway analysis of KRAS isoform-expressing Colo741 cell clones.
| System Gene Category – Term | Count | % | P-Value | Genes |
| KRASG12V Vs KRASWT | ||||
| GO biological process | ||||
| Cell cycle arrest | 6 | 6.7 | 5.50E-05 | DDIT3, DHCR24, GADD45A, IL8, PPP1R15A, SESN2 |
| Apoptosis | 13 | 14.6 | 3.90E-04 | ANXA1, APOE, GADD45A, IFIH1, IL24, DDIT3, DDIT4, DHCR24, PMAIP1, PPP1R15A, SEMA6A, TNFRSF19, TRIB3 |
| KEGG pathway | ||||
| Nitrogen metabolism | 3 | 3.4 | 1.50E-02 | ASNS, CTH, GLS |
| KRASG12D Vs KRASWT | ||||
| GO biological process | ||||
| Cellular component organization and biogenesis | 12 | 27.3 | 5.10E-02 | CRYAB, EHD2, FHOD1, HIST1H1A, HSPB1, LIN7C, MAP3K11, PCDHB5, PCDHB16, SEMA6A, SLC7A11, SMCHD1 |
| KRASG12D Vs KRASG12V | ||||
| GO biological process | ||||
| Immune system process | 14 | 32.6 | 3.50E-07 | BST2, CDK6, IFITM3, IFIT1, IFI27, IFI44, IL8, IL24, MICA, OAS1, OAS2, OAS3, SYK, S100B |
| Sterol metabolic process | 6 | 14 | 1.10E-06 | APOE, FDFT1, HMGCR, HMGCS1, IDI1, SC4MOL |
| KEGG pathway | ||||
| Biosynthesis of steroids | 4 | 9.2 | 5.70E-05 | FDFT1, IDI1, HMGCR, SC4MOL |
Gene name symbols used are those approved by the Human Genome Organisation Gene Nomenclature Committee http://www.genenames.org/.
Non-redundant functional categories, number of genes contained within each category, percentages ranked by the degree of over-representation in the category as determined by EASE (P-value) and gene members found to be modulated by the KRAS isoforms are shown. Redundant categories with similar gene members were removed to yield a single representative category.