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. 2009 Jan 12;184(1):173–183. doi: 10.1083/jcb.200801071

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© 2009 Johswich et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Activity of β4GalNAcTB is disrupted by Triton X-100. (A) Microsomal preparations of HEK293 transfected with β4GalNAcTs and GABPI as indicated were assayed for activity with GlcNAc-pNP as an acceptor and [³H]UDP-GalNAc or [³H]UDP-Gal (endogenous activity as an internal control) as donor substrates. Each value represents the mean of three independent vesicle preparations with standard deviation. GalNAcTB mix GABPI indicates that the proteins were expressed separately but mixed afterward for assays. (B and C) Microsomal fractions of HEK293 cells transfected with β4GalNAcTA (B) or the combination β4GalNAcTB–GABPI (C) were treated with Triton X-100 and saponin in various concentrations and assayed for GalNAcT activity as in A.