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. 2009 Jan 12;184(1):173–183. doi: 10.1083/jcb.200801071

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© 2009 Johswich et al.

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Figure 3. — The Golgi resident protein GABPI pilots β4GalNAcTB to the Golgi apparatus. The subcellular localization of β4GalNAcTs and GABPI was analyzed with N-terminally tagged proteins expressed in HEK293 cells. (A–C) Flag-β4GalNAcTA, -B, and Myc-GABPI were visualized by indirect immunofluorescence (red). Subcellular compartments were labeled with anti–α-mannosidase II (Golgi) or calnexin (ER; green). (D–F) Flag-β4GalNAcTB (red) coexpressed with Myc-GABPI (F, green). (G) Subcellular localization of Myc-GABPI containing an N-terminal KKTN sequence. (H) Flag-β4GalNAcTB (green) coexpressed with Myc-GABPI–KKTN (red). Nuclei were stained with Hoechst 33258 (blue).