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. 2009 Jan 12;184(1):173–183. doi: 10.1083/jcb.200801071

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© 2009 Johswich et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Knockdown of GABPI in S2 cells interferes with Golgi localization of β4GalNAcTB. (A–D) S2 cells after 3 d of culture in the absence (A and B) or presence (C and D) of dsRNA directed against GABPI were transiently transfected with Flag-β4GalNAcTA and HA-β4GalNAcTB. The Flag and HA epitopes were detected 2 d after transfection using respective antibodies, and the ER was marked with anti-HDEL. Nuclei were stained with Hoechst 33258.