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. 2009 Jan 12;184(1):143–158. doi: 10.1083/jcb.200807176

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Figure 4. — Cert^gt/gt embryos exhibit evidence of ER stress. (A) Western blot analysis of cell extracts of E10.5 wild-type and Cert^gt/gt embryos probed for proximal sensors such as phosphorylated PERK, IRE1-α, and ATF6 showing no or a slight increase in levels, a situation not different from cells in states of chronic stress. (B) Components of the UPR response implicated in the increased protein-folding efficiency of the ER such as ATF4, PDI, and calnexin were all increased in Cert^gt/gt embryonic extract. The increased levels of phosphorylated eIF2-α are an indicator of the underlying ER stress. +/+, wild type; −/−, homozygous mutant.