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. 2009 Jan 12;184(1):113–127. doi: 10.1083/jcb.200806044

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© 2009 Roberts-Galbraith et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — cdc15_ΔSH3 cells show defects in contractile ring dynamics. (A) rlc1-GFP and rlc1-GFP cdc15_ΔSH3-FLAG₃ cells were grown and imaged as in Fig. 1 D. BF, bright field. (B) rlc1-GFP and cdc15_ΔSH3-FLAG₃ rlc1-GFP cells were imaged live at 25°C every 2.5 min (Videos 1 and 2, available at http://www.jcb.org/cgi/content/full/jcb.200806044/DC1). Montages of cells completing cytokinesis are shown. Each image is 12.5 min apart. (C) cps1-191 rlc1-GFP and cps1-191 cdc15_ΔSH3-FLAG₃ rlc1-GFP cells were arrested and imaged. In the images, cells were divided into fifths, and the location of the contractile ring was quantified (n = 100). wt, wild type. (D) acyl-GFP was expressed in wild-type or cdc15_ΔSH3-FLAG₃ cells and imaged live. Membrane bridges remaining after cytokinesis are marked with arrows. Bars: (A and C) 5 μm; (B) 2 μm; (D) 3 μm.