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. 2009 Jan 12;184(1):101–112. doi: 10.1083/jcb.200805155

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© 2009 Wall et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — RBPJ-κ signaling is not required for Shh induction of Hes1 but is necessary for Shh induction of Hes5. Retinal explants were electroporated with shRBPJ-κ or a control short hairpin plasmid at P0 and cultured for 4 d with or without a Smo agonist. (a–c) GFP fluorescence localizes the transfected cells. ISH was performed for RBPJ-κ (d–f), Hes1 (g–i), and Hes5 (j–l). Bar, 100 μm. (m) Retinal explants were coelectroporated with an empty vector control, NICD, or Smo-M2 and pUB-GFP, and cultured for 3 d, then Hes1 expression was analyzed by RT-qPCR. Values represent the relative induction of Hes1 expression normalized to GFP. Error bars represent SEM. *, P < 0.005.