Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jan 12;184(1):101–112. doi: 10.1083/jcb.200805155

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Wall et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Shh-mediated RPC proliferation and cell fate specification requires Hes1. (a–c) In vivo anti-pH3 staining of the central retina adjacent to the optic nerve (asterisks) in P5 wild-type (Wt), PtchlacZ^+/−, and PtchlacZ^+/−Hes1^+/− retinas. Arrows indicate pH3-positive cells. Note that pH3+ cells in the vicinity of the optic nerve are rare in Wt and compound heterozygous mice. Bar, 100 μm. (d) Quantitative analysis of BrdU incorporation in vivo from P5 Wt (n = 3), Hes1^+/− (n = 3), PtchlacZ^+/− (n = 3), and PtchlacZ^+/−Hes1^+/− (n = 6) retinas. Values represent the mean number of BrdU-positive cells counted from three sections per animal. (e) Quantification of the proportion of BrdU⁺ cells in single-cell dissociates from the retinas of Wt (n = 5), Hes1^+/− (n = 3), PtchlacZ^+/− (n = 8), and PtchlacZ^+/−Hes1^+/− (n = 7) retinas at P5. (f) Retinal explants from Hes1^−/− (n = 3) or Wt (n = 3) animals were treated with a Smo agonist for 3 d, dissociated, and scored for the proportion of BrdU⁺DAPI⁺ cells. (g) Quantitative analysis for BrdU, CRALBP, Chx10, rhodopsin, and recoverin-positive cells in Smo agonist–treated P0 retinal explants electroporated with GFP and Hes1DN. Values are based on scoring marker+ cells among the transfected cohort in dissociates from retinal explants and represent the fold induction of double-positive (marker+GFP+) cells in GFP + Ag and Hes1DN + Ag cultures compared with double-positive cells in GFP-transfected untreated explants. There is no difference in proliferation or cell type composition in GFP and Hes1DN-transfected cells in untreated explants. Error bars represent SEM. *, P < 0.05; **, P < 0.01.