Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jan 12;184(1):67–82. doi: 10.1083/jcb.200801009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Zhang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — Characterization of NANOG-overexpressing hESC sublines. (A) Quantitative RT-PCR for endogenous (right bars) and total (left bars) expression of NANOG in H1, H9, and hES-NCL1 sublines. The data represent the mean ± SEM (error bars) from four independent experiments. The value for the control clone from each hESC subline was set to 1, and all other values were calculated with respect to this. (B) Western blot showing NANOG and OCT4 expression in the H9 sublines. β-Actin was used as a loading control. H9, wild-type untransfected cells; H9C, H9 control subline; H9N, H9 NANOG-overexpressing subline. Molecular masses are indicated in kilodaltons. (C) Assessment of cell death in NANOG-overexpressing and control sublines by flow cytometry analysis. (D) Assessment of cell proliferation in NANOG-overexpressing and control sublines by flow cytometry after 45 min of BrdU incorporation. (C and D) The data represent the mean ± SEM from three independent experiments. (E) Flow cytometry images showing a higher percentage of proliferating cells (gate R2) in NANOG-overexpressing sublines compared with controls.