Fig. 6.

The most common mutations of CLN3 can rescue the vacuole size defect, but have minimal effects on other phenotypes. (A-D) Phenotypes of btn1Δ cells expressing the indicated mutant GFP-Btn1p proteins: (A) mean vacuole diameter (μm); (B) mean septation index (% of total cells); (C) mean percentage of monopolar cells (% of total septated cells after 7 hours at 37°C); (D) mean percentage of curved or bent cells (% of total cells after 4 hours at 37°C). Dotted line=mean value for btn1Δ cells, unbroken line=mean value for btn1Δ cells + GFP-Btn1p. (Data shown is the mean±s.d. of at least three independent experiments; ***P<0.001, **P<0.01, *P<0.05.)