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. 2008 Oct 29;28(44):11421–11431. doi: 10.1523/JNEUROSCI.2873-08.2008

Figure 4.

Figure 4.

Cyclin D1 expression in neuronal cultures derived from PS1 mutants. A, Representative images of NeuN and Cyclin D1 immunostaining of primary neuronal cultures isolated from E15.5 of various PS mutants. Ctrl, PS1+/+ or PS1+/− (combined as no differences could be detected); PS1−/−, PS1 knock-out; PS1−/− hPS1, PS1 knock-out expressing wild-type human PS1; PS1−/− D257A, PS1 knock-out expressing D257A mutant of human PS1; PS1 ΔE10, PS1 knock-in mice with deletion of exon 10. Neuronal nuclei were marked with an anti-NeuN antibody. NeuN/Cyclin D1 double-positive cells could be found in PS1−/− and PS1 ΔE10 neurons but not in PS1−/−; hPS1 or PS1−/−; D257A neurons. Scale bar, 10 μm. B, Quantification of percentage of neurons positive for Cyclin D1. Approximately 1000 cells were counted from five independent experiments. ***p < 0.001, Student's t test. C, Representative Western blotting of Cyclin D1 levels in various PS cultures. γ-Tubulin was used as a loading control.