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. 2008 May 27;69(1):164–174. doi: 10.1111/j.1365-2958.2008.06274.x

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© 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd

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Fig. 3 — Lipid analysis of planktonically grown cells and biofilms of M. tuberculosis. Total apolar and polar lipids from planktonic and biofilm cultures of M. tuberculosis mc²7000 resolved on 2D-TLC are solvent systems as described in the Experimental procedures (solvent A, Sol. A; solvent C, Sol. C; solvent D, Sol. D). Samples were labelled with ¹⁴C-acetate and equal amounts of total radioactivity were examined. The lipids are annotated as previously described (Besra, 1998): A–C, phthiocerol dimycoserosate family; MK, menaquinone; D and E, apolar mycolipenates of trehalose; F, free fatty acid; CF, cord factor; DAT, 2,3,-di-O-acyltrehaloses; SL, sulphatides. Spot 1 which is induced in biofilms is also marked.