Table 2. The amino acid¹⁹⁵, RT-PCR and specific activity of PAF-AH in ten leptospires cultured in EMJH and Korthof media.

Strain	Amino acid195	RT-PCR a	RT-PCR b	Specific activity c (IU/1010 leptospira)	Specific activity d (IU/1010 leptospira)
L. interrogans serovar Lai strain 56601	Tyr	+	+	0.22±0.023	0.4±0.045
Avirulent variant strain of L. interrogans serovar lai	Tyr	+	+	0.18±0.015	0.38±0.05
L. interrogans serovar Javanica strain M 10	Tyr	+	+	0.20±0.021	0.48±0.06
L. interrogans serovar Canicola strain Lin	His	+	+	0.15±0.018	0.45±0.06
L. interrogans serovar Pomona strain Luo	His	+	+	0.14±0.017	0.35±0.03
L. interrogans serovar Linhai strain Lin 6	Tyr	+	+	0.17±0.023	0.40±0.05
L. interrogans serovar Hebdomadis strain P 7	His	+	+	0.25±0.03	0.37±0.03
L. interrogans serovar Paidjan strain L 37	Tyr	+	+	0.24±0.03	0.34±0.03
L. biflexa serovar Montevalerio	Tyr	+	+	0.28±0.025	0.48±0.05
L. biflexa serovar Anhui strain Zong 7	His	+	+	0.21±0.016	0.41±0.06

^aRT-PCR detection of l-paf-ah mRNA from leptospires cultured in EMJH medium. +, positive result.

^bRT-PCR detection of l-paf-ah mRNA from leptospires cultured in Korthof medium. +, positive result.

^cSpecific activity of leptospires cultured in EMJH medium.

^dSpecific activity of leptospires cultured in Korthof medium.