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. 2009 Jan 16;4(1):e4213. doi: 10.1371/journal.pone.0004213

Figure 3. Regional gene expression is maintained in vitro and is cell intrinsic.

Figure 3

RT-PCR of Emx2 and Hoxd10 expression in E14 cortical and spinal cord tissue and neurospheres at first and 13th passage (clonal density). Emx2 and Hoxd10 expression in E14 cortical and spinal cord neurospheres that had been cultured in media that was conditioned by neurospheres from the opposing region and passaged 1 and 6 times (conditioned media).