Table 2. Confirmation of differential gene expression based on region by qRT-PCR.

		Ratio from Microarray	E14 secondary spinal cord NS relative to E14 secondary cortical NS	E14 13th passage spinal cord NS relative to E14 13th passage cortical NS	E14 secondary spinal cord NS relative to E11 secondary cortical NS	E14 secondary spinal cord NS relative to E17 secondary cortical NS
Cortical Enriched Genes	Lhx2	0.149	0.108	0.485	0.076	0.053
	Nr2e1	0.019	0.001	0.038	0.003	0.002
	Emx2	0.129	0.012	0.166	0.005	0.898
	Arx	0.28	0.524	0.527	0.499	0.223
	Egf-R	0.292	0.006	0.093	0.002	1.072
	Ntrk2	0.416	0.543	0.484	0.508	0.278
	Ccng1	0.445	0.817	0.442	0.402	1.159
Spinal Cord Enriched Genes	Hoxc10	5.221	634.448	678.059	367.427	470.030
	Pace4	5.212	2.480	0.708	12.569	1.591
	Irx3	3.21	21.470	4.846	22.765	8.692
	Anxa2	2.596	3.447	0.506	1.217	1.794
	Abcg2	2.45	5.202	2.100	2.395	4.658
	Cav2	2.409	2.549	3.174	4.329	1.537
	Fut9	2.225	2.908	3.401	1.295	0.174
	Mro	2.002	1.905	1.161	2.487	1.078

RNA was extracted from neurospheres derived from E14 cortex and spinal cord and E11 and E17 cortex. The following comparisons were made: Secondary E14 cortical and spinal cord derived neurospheres, E14 cortical and spinal cord neurospheres after 13 passages, E11 cortical derived and E14 spinal cord derived neurospheres, E17 cortical and E14 spinal cord derived neurospheres. Results represent the delta delta critical threshold of cortical derived neurospheres compared to spinal cord derived neurospheres. Genes that maintain their differential expression between cortical and spinal cord neurospheres through all conditions are in bold. The first seven genes were identified by microarray as enriched in cortical derived neurospheres. The bottom eight genes were identified by microarray as enriched in spinal cord derived neurospheres. Relative expression>1 indicates greater expression in spinal cord derived neurospheres.