Skip to main content
. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Anal Chem. 2008 Oct 3;80(21):8231–8238. doi: 10.1021/ac801317t

Figure 7.

Figure 7

A) Illustration of desegmenting injection. Top drawing shows the lay-out of channels with addition of sampling channel and cross-style injector. Drawing illustrates a sample plug coalescing and portion being transferred to sampling channel for injection and separation. Below are two sequential fluorescence micrographs showing coalescence of a fluorescein plug to the virtual wall illustrating “residence time”. Times (t) indicate time since the plug arrived at the interface. The plugs were pumped past the coalescence interface at 5 μL/min, with a linear velocity of 0.42 cm/s. The cross flow separation buffer was pumped at a rate of 40 nL/min, with a linear velocity of 0.03 cm/s. B) Sequential fluorescein injections performed by sampling from a K interface with a cross flow of 40 nL/min and a 1 Hz segmented flow stream. C) Effect of cross flow rate on residence time in the sampling channel.