Panel A) A light blue hue develops in the sample containing methanol at a concentration of 1 mg/dl. The intensity of the color increases proportionately with the increase in methanol concentration from 1 mg/dl to 100 mg/dl. WB, water negative control; SB, saliva negative control. Panel B) The alcohol oxidase method was used to detect methanol (MeOH), ethanol (EtOH), isopropanol (i-PrOH), ethylene glycol (EG), and diethylene glycol (DEG) in saliva. A strong blue hue was noted with methanol as before. A blue hue developed with ethanol at a concentration of 50 mg/dl. Isopropanol (i-PrOH), ethylene glycol (EG), and diethylene glycol (DEG) were not detected by this method even at concentration of 100 mg/dl.