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. Author manuscript; available in PMC: 2009 Jan 8.
Published in final edited form as: Cell. 2008 Apr 18;133(2):250–264. doi: 10.1016/j.cell.2008.03.028

Figure 4. Marker expression and promoter methylation analysis of B-iPS lines.

Figure 4

(A) Immunoflorescence staining for ES pluripotency markers Oct4, Nanog (signal obtained from GFP cassette knocked in the endogenous Nanog locus), SSEA1 and AP. (B) RT-PCR analysis of selected ES marker genes in B cells, ES cells, MEF-iPS cells and different iB-iPS and B-iPS lines. (C) Analysis of the methylation state of the Oct4 and Nanog promoters using bisulphate sequencing. Open squares indicate unmethylated and filled squares methylated CpG dinucleotides. (D) Real-time PCR after chromatin immunoprecipitation using antibodies against H3K4me3 and H3K27me3. Shown are the Log2 enrichments for several previously reported “bivalent” loci in ES cells.