Fig. 5.

FACS analysis of cardiac lineage markers, Nkx2.5 and αMHC, in BMP2-induced differentiated cells from plated EBs. As high as 73.6% of differentiated cells were positive for both cardiac markers, evaluated 20 days after plating (Suppl. Fig. 1). Moving image of hESC-derived cardiomyocytes from long-term cultured EBs treated with BMP2. Images were recorded under phase contrast microscope (× 200), and contractile region showed regular contractions with a frequency of 1 beat/second.