Skip to main content
. 2008 Dec 10;28(50):13532–13541. doi: 10.1523/JNEUROSCI.0847-08.2008

Figure 5.

Figure 5.

mGlu5 activation produces a persistent CB1-dependent hyperpolarization that mimics SSI in LTS interneurons. A, Current-clamp recording of an LTS interneuron in the constant presence of the Na+ channel blocker tetrodotoxin (TTX, 0.5 μm; white horizontal bar). Local perfusion of the mGlu5 specific agonist CHPG (1 mm, gray horizontal bar) produced a depolarization followed by a persistent hyperpolarization when the drug was washed out. Horizontal dashed line: resting Vm (−61 mV). B, Summary plot of changes of membrane potential (left) and conductance (right) at the time points (a, b) indicated in A. C, Current-clamp recording of another LTS interneuron in the constant presence of TTX and the CB1 antagonist AM251 (2 μm). mGlu5 activation (gray bar) depolarized the cell with no apparent hyperpolarization following CHPG washout. Horizontal dashed line: resting Vm (−62 mV). D, Summary plot of changes of membrane potential and conductance at the time points (a, b) indicated in C. E–H, Similar experiment as in A-C in the continuous presence of the PLC blocker U-73122 (E–F) or the DAGL inhibitor THL(G–H). ***p < 0.001; *p < 0.05; ns: not significantly different, Wilcoxon signed-rank test.