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. Author manuscript; available in PMC: 2009 Dec 10.
Published in final edited form as: Neuron. 2008 Dec 10;60(5):775–787. doi: 10.1016/j.neuron.2008.09.040

Figure 3. BRG1 recruits HDAC and Rb to inhibit c-fos activation.

Figure 3

A. Relative CAT activity in E18 cortical neurons transfected with mouse -350 c-fos-CAT reporter along with shRNA against GFP and rat BRG1 at 3DIV, and stimulated as indicated at 5 DIV (KCl 50 mM).

B. Relative CAT activity in rat E18 cortical neurons transfected with mouse -350 c-fos-CAT reporter along with b-galactosidase and wild type BRG1 constructs at 3DIV, and stimulated as indicated at 5 DIV (KCl 50 mM) with the presence of DMSO.

C. Relative CAT activity in rat E18 cortical neurons transfected with mouse -350 c-fos-CAT reporter along with b-galactosidase and wild type BRG1 construct at 3DIV, and stimulated as indicated at 5 DIV (KCl 50 mM) with TSA pre-treatment (TSA 250ng/ml).

D. Immunoprecipitation of BRG1 with class I HDAC complex. Cortices from postnatal 4 day ratx were dissected, homogenized and immunoprecipitated with antibodies as indicated. The immunoprecipitates were resolved by SDS-PAGE and the Western blot was probed with an anti-BRG1 antibody.

E. Immunoprecipitation of BRG1 with Rb. Nuclear fractions of rat E18 cortical neurons were collected at 5DIV, and immunoprecipitated with IgG and anti-Rb antibody. The immunoprecipitates were resolved by SDS-PAGE and Western blot was probed with an anti-BRG1 antibody.

F. Immunoprecipitation of Rb with HDAC1. Nuclear fractions of rat E18 cortical neurons were collected at 5DIV, and immunoprecipitated with IgG and anti-HDAC1 antibody. The immunoprecipitates were resolved by SDS-PAGE and Western blot was probed with an anti-Rb antibody.

G. Relative CAT activity in rat E18 cortical neurons transfected with mouse -350 c-fos-CAT reporter along with b-galactosidase, wild type BRG1 and mutated BRG1 (LTCEERTREE) constructs at 3DIV, and stimulated as indicated at 5 DIV (KCl 50 mM).

H. Relative CAT activity in rat E18 cortical neurons transfected with mouse -350 c-fos-CAT reporter along with b-galactosidase, wild type Retinoblastoma protein and mutated Rb protein (Δexon 22) constructs at 3DIV, and stimulated as indicated at 5 DIV (KCl 50 mM).

Asterisks indicate significance at p<0.05. Error bars represent +SD.