Table 1.
Line | Age (weeks) | Thymocytes (×106) | % Thymocytes (DP) CD4+/CD8+ | % Thymocytes Vβ8.2high/CD4+ | % T cells (LN) Vβ8.2+/CD4+ |
---|---|---|---|---|---|
B10.PL | 6 | 176.0 | 83.8 | 3.8 | 9.6 |
Tg4a | 5 | 85.9 | 57.0 | 20.7 | 48.6 |
9 | 150.4 | 70.9 | 16.0 | 30.3 | |
12 | 54 | 68.2 | 20.7 | 45.8 | |
6 | 6.7 | 0.4 | 1.7 | n.d.b | |
9 | 1.4 | 0.1 | 9.0 | 13.0 | |
12 | 1.4 | 0.1 | 5.9 | 4.8 | |
13 | 0.8 | 1.1 | 9.0 | 3.5 | |
5 | 28.4 | 52.2 | 1.3 | 5.5 | |
9 | 1.2 | 51.7 | 6.1 | 3.8 | |
11 | 4.0 | 9.5 | 3.8 | 9.4 |
Thymocytes from the respective mouse lines of individual animals with the indicated ages were prepared, counted and stained against CD4, CD8 and Vβ8.2 and then analyzed by FACS. The total thymocyte number (cellularity), which is dependent on the age as well as on the expression of the transgenic constructs, is shown in the third column in millions. Columns 4-6 show the relative percentages of the indicated cell populations determined by FACS (of which examples are shown in Fig. 3). Data from individual mice are shown.
Single transgenic littermates of F1-double transgenics.
n.d.: not determined.