unc-94/tmd-1(RNAi) enhances formation of actin aggregates in profilin mutant backgrounds. (A) Frequency of adult worms that have actin aggregates in the body wall muscle. Adult worms were stained with tetramethylrhodamine-phalloidin, and worms with actin aggregates in their muscle were scored. Wild-type, pfn-2(null) and pfn-3(null) single mutants, and a pfn-3 pfn-2 double mutant were treated with control RNAi or unc-94/tmd-1(RNAi). (B) Actin filament organization in the body wall muscle of wild-type (a and b), pfn-2(null) (c and d), pfn-3(null) (e and f) and pfn-3(null) pfn-2(null) (g and h) treated with control RNAi (a, c, e, and g) or unc-94/tmd-1(RNAi) (b, d, f, and h). Bar, 10 μm.