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PMCA and NHERF-2 in HT-29 cells. A, X-Y scans of pan-PMCA (panel i) and NHERF-2 (panel ii) revealing punctuate distribution and some degree of co-localization (panel iii). B, Z-scan of HT-29 cells showed an apical-lateral distribution of both PMCA (panel i) and NHERF-2 (panel ii) with a significant degree of colocalization (panel iii). Scale bar represents 10 μm. These images are representative of four separate experiments.
