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. 2009 Jan 16;284(3):1820–1830. doi: 10.1074/jbc.M804590200

FIGURE 8.

FIGURE 8.

Depletion of Ca2+ stores triggers PMCA and NHERF-2 trafficking to the cell membrane. HT-29 cells were exposed to CPA (5 μm) in the absence of extracellular Ca2+. A, representative Fura-2 tracing showing changes in [Ca2+]i following the addition of CPA (5 μm) to the bathing solution at 20 s. B, representative Western blot showing changes in membrane-associated PMCA during exposure to CPA. C, densitometry showing time-dependent changes in membrane-associated PMCA during exposure to CPA. Membrane levels of PMCA peaked to 61 ± 12% (n = 3; **, p < 0.01) above control levels at 120 s and returned to baseline levels by 300 s. D, representative Western blot showing changes in membrane-associated NHERF-2 during exposure to CPA. E, densitometry showing changes in membrane-associated NHERF-2 during exposure to CPA. Membrane levels of NHERF-2 peaked by 180 ± 11% (n = 3; *, p < 0.05; **, p < 0.01; ***, p < 0.001) and returned to resting levels by 300 s. All data are expressed as mean ± S.E.