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. 2009 Jan 16;284(3):1612–1619. doi: 10.1074/jbc.M806981200

FIGURE 7.

FIGURE 7.

Effect of VacA on β-catenin-Tcf-mediated transcription and cyclin D1 promoter activity. AZ-521 cells were transfected with the luciferase reporter plasmid TOPtkLuciferase (filled bars) or FOPtkLuciferase (open bars), and internal control pRL-TK, followed by incubation with 120 nm VacA (A) or iVacA (B) for indicated times. Luciferase activities were measured using the Dual-Luciferase Reporter Assay System. Relative luciferase activities were compared with activities obtained for TOPtkLuciferase at 0 min in the presence of toxin. Data are means ± S.E. of values from triplicate experiments, with an n = 3 per experiment. Statistical significance: *, p < 0.01. C, AZ-521 cells were transfected with a reporter plasmid containing the cyclin D1 promoter-luciferase gene or a control empty vector. After transfection, cells were incubated with VacA (filled bars) or iVacA (open bars). Relative luciferase activities were compared with activities obtained at 0 min in the presence of iVacA. Data are means ± S.E. of values from triplicate experiments, with an n = 3 per experiment. Statistical significance: *, p < 0.01.