Effect of VacA on β-catenin-Tcf-mediated transcription and cyclin
D1 promoter activity. AZ-521 cells were transfected with the luciferase
reporter plasmid TOPtkLuciferase (filled bars) or FOPtkLuciferase
(open bars), and internal control pRL-TK, followed by incubation with
120 nm VacA (A) or iVacA (B) for indicated times.
Luciferase activities were measured using the Dual-Luciferase Reporter Assay
System. Relative luciferase activities were compared with activities obtained
for TOPtkLuciferase at 0 min in the presence of toxin. Data are means ±
S.E. of values from triplicate experiments, with an n = 3 per
experiment. Statistical significance: *, p < 0.01.
C, AZ-521 cells were transfected with a reporter plasmid containing
the cyclin D1 promoter-luciferase gene or a control empty vector. After
transfection, cells were incubated with VacA (filled bars) or iVacA
(open bars). Relative luciferase activities were compared with
activities obtained at 0 min in the presence of iVacA. Data are means ±
S.E. of values from triplicate experiments, with an n = 3 per
experiment. Statistical significance: *, p < 0.01.