Figure 4.

Effects of antisense deoxyoligonucleotides that disrupt pyr AT1 on termination of transcription in vitro. Template 1 was used for transcription (see Materials and Methods), and the radioactive transcripts were analyzed by electrophoresis. Components added to each reaction are shown (Top). Numbers for Oligo indicate the molar excess (fold) of deoxyoligonucleotide over template DNA. Numbers at the bottom of each lane indicate the mol % of terminated transcripts. RT, read-through transcripts; T, terminated transcripts.