Table 1.
Effects of antisense deoxyoligonucleotides on termination of transcription from B. subtilis pyr templates in vitro
| Oligo added | % termination
|
||||
|---|---|---|---|---|---|
| −PyrR, −UMP | +PyrR, +UMP | −PyrR, −UMP
|
|||
| [Oligo]/[Template]
| |||||
| 10 | 100 | 1000 | |||
| Template 1 | |||||
| None | 32 ± 12 | 91 ± 5 | – | – | – |
| T11 | 35 | 37 | 46 | ||
| T12 | 35 | 32 | 13 | ||
| Template 2 | |||||
| None | 67 ± 4 | 94 ± 3 | – | – | – |
| AT21 | 71 | 90 | 96 | ||
| AT22 | 62 | 62 | 67 | ||
| T23 | 63 | 63 | 67 | ||
| T24 | 60 | 44 | 7 | ||
| BL22 | 61 | 23 | 4 | ||
| BL23 | 66 | 67 | 64 | ||
| BL24 | 59 | 30 | 7 | ||
| BL25 | 67 | 68 | 71 | ||
| BL27 | 67 | 66 | 45 | ||
| BL28 | 67 | 61 | 15 | ||
| BL23 + BL25 | ND | ND | 41 | ||
Oligo, oligonucleotide; ND, not determined. The numbers 10, 100, and 1000 give the molar ratio of deoxyoligonucleotide to DNA template in the experiment.