FIGURE 5.

Lack of G activation results in inability to trigger F. A, CHO cells expressing F and G were mixed with either CHO cells (negative control) or CHOB2 cells for 2 h at 4 °C. Cell mixtures were brought to 37 °C or kept at 4 °C for 45 more min to allow for triggering of F or not in the presence of 500 nm biotinylated HR2 peptide. B, the MFI values are summarized for 4 or 37 °C and in the absence (-B2) or presence (+B2) of B2 for the F/G (WT), F/4-4, and F/4-5 protein pairs. Data are the average ± S.E. are shown, n = 3. The p value for the MFI of the (+B2) versus (-B2) conditions at 37 °C for WT F/G was 0.02.