FIGURE 5.

Deletion mutagenesis reveals a key domain in the p115 CTF that confers the apoptotic phenotype. A, schematic representation of various GFP- and FLAG-tagged constructs of human p115 CTF. The positions of the coiled-coil (CC) domains 2, 3, and 4 are indicated as well as that of the C-terminal acidic GM130/giantin binding region. B, COS-7 cells were transfected with GFP-tagged CTF constructs: p115-(758–859) (panels A–D), p115-(884–962) (panels E–H), and p115-(859–962) (panels I–L). Cells were fixed after 18 h of transfection, and stained with a monoclonal antibody to GM130 (red) and Hoechst 33238 (blue). Transfected cells were identified by GFP fluorescence (green). Bar, 10 μm. Similar results were obtained using FLAG-tagged constructs (data not shown).