TABLE 1.

Hydrodynamic properties of recombinant ASAP1 determined by sedimentation velocity experiments BAR-PZA and BAR-PH are the homology models shown in the Fig. 1A. BAR_PZA assumes a bead-on-a-string arrangement for the BAR and PZA segments of ASAP1. BAR_PH assumes a bead-on-string arrangement for the BAR and PH domains. BARP_ZA assumes that the BAR and PH domains form a bead and that ZA forms a second bead. BAR_P_ZA assumes that BAR, PH, and ZA are each beads on the string.

Protein	swa	s20,wb	shc	f/fod	Axial dimensionse
BAR-PZAf	5.1	5.9	6.6	1.82	32.8 × 3.3
BAR_PZA			5.9
BARP_ZA			5.9
BAR_P_ZA			5.3
BAR-PHf	3.9	4.5	4.8	1.73	24.9 × 3.0
BAR_PH			4.2
PZA	2.8	3.2	3.6	1.44	11.9 × 2.9
ZA	2.4	2.7	2.9	1.36	8.8 × 2.9

^aWeight average sedimentation coefficient at experimental conditions

^bCorrected to standard conditions (water, 20 °C)

^cSedimentation coefficient determined for homology model using Hydropro7

^dFrictional ratio obtained with s_20,w and molar mass using SEDNTERP

^eAxial dimensions (nm) of cylinder model (length × diameter) assuming hydration level of 0.3 g of water/gram of protein

^fThese domains were present exclusively as dimers in solution above 0.1 mg/ml (1 μm)