TABLE 2.
Effect of vesicle size and composition on the C50 values of PZA and BAR-PZA C50 values were determined as described previously (26). DSPC indicates LUVs containing 55% DSPC, 20% PE, 15% PS, 7.5% PI, and 2.5% PIP2. Egg PC indicates LUVs containing 55% egg PC, 20% PE, 15% PS, 7.5% PI, and 2.5% PIP2. 1 μm indicates LUVs were extruded through a filter with a 1-μm pore; 0.1 μm indicates extrusion through filters with a 0.1-μm pore. The results are the means ± S.E. of three experiments.
Enzyme
|
C50
|
||||||
---|---|---|---|---|---|---|---|
DSPC, 1 μm | DSPC, 0.1 μm | Egg PC, 1 μm | Egg PC, 0.1 μm | ||||
nm | |||||||
PZA | 0.017 ± 0.005 | 0.031 ± 0.004 | 0.076 ± 0.014 | 0.075 ± 0.016 | |||
BAR-PZA | 1.00 ± 0.37 | 1.24 ± 0.05 | 0.90 ± 0.17 | 0.89 ± 0.09 |