FIGURE 7.

Phospholipid analysis of ER membrane in palmitate-treated cells. CHO cells were cultured for 24 h in LPDS medium in the presence and absence of 50 μm palmitate. ER-enriched fractions were prepared, and lipids were extracted. Electrospray ionization-mass spectrometry was performed, and lipid species were analyzed. Data are shown for PC (A), phosphatidylglycerol (PG) (B), and phosphatidylinositol (PI) (C) content of the ER membranes. Values are means ± S.E. ^*, p < 0.05 for palmitate treatment versus LPDS.