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Spontaneous strand exchange at elevated temperatures. Direct and reverse strand exchange reactions were performed in conditions identical to those used for RecA-promoted reactions but in the absence of the protein and at elevated temperatures, 41 °C (A) and 45 °C (B). Kinetic curves for reverse (1 and 3) and direct (2 and 4) strand exchange are presented for the pair of completely homologous (1 and 2) and mismatch containing (3 and 4) substrates.
