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Strand exchange between short oligonucleotides promoted by human recombinases. A, kinetic curves for Rad51 promoted (1 and 2) and spontaneous (3), direct (1) and reverse (2 and 3) strand exchange reactions. All oligonucleotide substrates are in equimolar amount. B, kinetic curves for Dmc1 promoted direct (1) and reverse (2 and 3) strand exchange at an equimolar ratio of oligonucleotide substrates (1 and 2) and with a 3-fold excess of the +16-mer (3).
