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. 2009 Jan 16;284(3):1514–1522. doi: 10.1074/jbc.M806994200

FIGURE 3.

FIGURE 3.

Pharmacological inhibition of PKC attenuates βAR enhancement of CICR. A, IP3R inhibition with 2-APB (20 μm, 5 min pre-treatment) does not inhibit βAR-dependent increases in Ca2+ transient amplitude (Δ405/485). Ca2+ transients were measured in the absence and presence of 1 μm isoproterenol. Data are pooled from 10 to 15 cells per treatment condition. Results are average (±S.E.). B and C, PKC inhibition (1 μm BIM, 5 min pre-treatment) significantly blunts (B) isoproterenol- and (C) cpTOME-induced increases in Ca2+-transient amplitude in PLCε+/+, but not PLCε–/– cardiac myocytes. BIM did not affect naïve Ca2+ transient amplitude. Data were pooled from 20–40 cells for each treatment condition from n = 3 PLCε+/+ and n = 2 PLCε–/– mice. Results are average (±S.E.); ***, p < 0.001; #, p < 0.001 for PLCε–/– response compared with PLCε+/+; ns is not significant; one-way ANOVA, Bonferroni post test.